March 14th

On Monday March 14th, I finished the CLPA Analysis for the DU145. Had I not used excel, this would have been extremely long, but thanks to the magic of excel it only took me an hour. This test measures expression of genes that respond to radiation exposure relative to the expression of genes that do not respond to radiation. I used an excel file to complete the analysis. The process is extremely long and complicated so I will not bore you with it. I will, however, love to share the results with you.

Here is the final graph:

On the x-axis are the the 8 genes that we analyzed. There are duplicates of each gene because there was a duplicate of the experiment. The housekeeping genes, the genes that are required for the basic functions of the cell, are MSRP18, CDR2, and MRPS5. All the other genes were normalized based on these average of these genes. 

The goal of the CLPA Assay is to determine the difference in gene expression between non-irradiated, single dose radiation, and fractionated dose radiation. 

To fully analyze these results, a biostatistical analysis must be conducted. This Dr. Lacombe will be doing this. Once this is done I will give further information on the significance of these results. 

In addition to the CLPA Assay I also worked in the cell culture room. We changed the medium in the 22Rv1 flasks and passed the DU145 cells to new flasks.