Thursday and Friday of the fifth week were simple. There only two things that I can be doing at any moment- lab work or sitting at my desk doing other work on my computer.
On Thursday March 10th, in the cell culture room we passed the PC3 cells into a new flasks (same as trypsinizing, froze and passed the 22Rv1 cells, and changed the medium in the DU145.
Just a quick tangent- Since I have been learning these procedures and protocols through hands on practice and direct presentation I do not know the proper names for all the procedures. Not to say that all the names I have stated are incorrect, at least 95 % are accurate, but there a few protocols that may not have a proper name. Basically, please bear with me as I continue to learn.
Back to the 10th. I have not mentioned this before because this project does not pertain to my senior research project, but I am also working on. Dr. Lacombe is working on creating a cartridge that can lyse a sample of cells and analyze the proteins. Currently, Dr. Lacombe is working out the portion of the cartridge that lyses the cells. For this project we conducted a micro-BCA protein assay.
On the 11th, we conducted a electrophoresis for the proteins form the micro-BCA protein assay. This was also for the cartridge. We also went to the cell culture room. There we changed the medium in flasks and passed cells.
Hi, Nandini! For the lay person, can you explain what it means to lyse a sample of cells and analyze the proteins? I am really impressed with all the hands-on lab work and data analysis you are conducting!
ReplyDelete