Friday, April 29, 2016

Here's the data! (well the first part of it)

The main event of this meet was the gene analysis. The differences in gene expression between the single dose and fractionated dose treatments is the main focus of this study.

The CLPA analysis and CE anlysis are the methods we used to analyze the genes. Here are the results:



I apologize for the lack of labels. Here's what you need to know:
  • Starting from the left: the first three columns (0,SD, and FD) are 22Rv1
  • The middle three (0, SD, and FD) are DU145
  • The last three (0,SD, and FD) are PC3
That's great, but what does it mean?

Don't worry! I'm here to explain. 

The panel on the bottom is a legend. ( For everyone who thought of Drake when they read that sentence...same). The green (0) indicates that the gene is not expressed  and the red indicates that the gene is over expressed. 

For the three cell lines, there is a similar gene profile. The genes are expressed and the gradient expression is relatively similar. 

There are 6 genes that are not expressed in any of the prostate cancer cell lines. This is indicated by the light green on the graph. These genes are CD27, CEBPB, PRIM1, BCL11B, PTRCAP, and LAPTM5. 

There are three genes that are over-expressed in the three cell lines. This is indicated by the red or black on the graph. These three genes are RFC4, PARP1, and HIST1H3D. 

On the graph, there is also an indication of some differences in expression between the single dose and fractionated dose treatments for some genes. To determine if these differences are significant, we must do a biostastic. 

What's that you say?

Well stay tuned to find out!









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